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This concentration was chosen as an effective working concentration; a higher or lower working concentration would reduce the accuracy due to the difficultly in counting the number of spermatozoa in the field of view if too low or high. Sperm chromatin structure assay is useful for fertility assessment. Bioavailability and bioefficacy of polyphenols in humans. In the case of unequal variances, determined by the test of homogeneity of variances, Tunnett T3 post-hoc analysis was used. Gonadal function in male patients with ankylosing spondylitis. Written informed consent was obtained from the participants before recruitment.